Physical Stability of Sea Buckthorn Oil Nanoemulsion with Tween 80 Variations

Sea buckthorn oil (SBO) contains fatty acids that have an anti-aging effect. The delivery system ofSBO can be improved by a nanoemulsion system. purpose of this study was to determine thephysical stability SBO (NSB) with Tween 80 variation. NSB made 1% SBOand variation 35% (FI); 40% (FII) and 45% (FIII). stored in climatic chamberat 30 ± 2°C RH 65%±5% for 90 days. Samples were taken before storage on the 15th, 30th,60th, 90th Evaluations carried out including appearance, particle size, polydispersityindex (PI), transmittance percentage (TP), pH, viscosity. Quantitative data analyzed withmultivariate analysis. result showed formulas yellowish-orange clear. Thedroplet size FII FIII stable (p > 0.05) range 12-15 nm PI < 0,5. All formulashad TP 95% pH 7.50–7.6. viscosity all 1.2-1.9 dPa.s characterizedas Newtonian. Increasing concentration tween reduce droplet PI. at aconcentration 35-45% produces sea which is days storage.